Data-enriched characterization of mobile populations. Morphological info from photos can add into the richness of flow cytometry facts. Such as, the figure shows an in any other case common apoptosis assay utilizing Annexin V and PI, adding mobile imaging to characterize cells in Each individual population to reveal morphologically distinct characteristics. These insights could not are received from multiplex staining alone.

As samples are acquired around the Attune CytPix Flow Cytometer, the substantial-velocity brightfield digicam captures and merchants photographs of detected functions at a charge as many as 6,000 visuals for every second, depending on the flow price and impression dimension. For higher versatility, Attune Cytometric Computer software allows you to modify the impression capture frequency as wanted and select a particular gate for impression capture, if demanded.

Incorporate acoustic concentrating technologies with higher-parameter Evaluation for flexible spectral unmixing and standard workflows.

Explore new insights into your experimental facts by visualizing populations working with combined fluorescence and morphology info. Our software program interface wizards permit downsampling, concatenation (merged data files) and dimensionality applications which have been developed to the software for an intuitive, streamlined details Assessment practical experience.

"A picture is just not pretty really worth a thousand dots, but it seriously helps to see visuals. It is great to check out photographs that correlate with staining. One example is, AnnexinV-good cells that happen to be morphologically diverse than wholesome cells, or CD14+ monocytes that are greater plus more textured than CD3+/CD4+/CD14- lymphocytes.

Additionally, it truly is illuminating to find out how many doublets slip by way of conventional singlet gates, and good to confirm the position of sudden double-optimistic gatherings in my analysis."

Pair your Attune flow cytometer using an autosampler for enhanced workflow effectiveness, and insert robotic integration for optimum throughput.

Dependable impression top quality even at significant throughput Acoustic focusing in addition to a higher-pace digital camera Blend to impression these T cells continually at reduced or higher flow fees. Easily change concentrate and digicam options to fulfill experimental requirements.

Early indications of this contamination had initially been dismissed as debris. Backgating to find new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also enables you to use morphological functions to find fascinating subpopulations that will not be obvious from flow cytometry knowledge on your own.

Customers can perspective event photographs in actual-time to confirm sample high-quality, enhance protocols, and history the morphology of each mobile population discovered for analysis. Imaging capture also Positive aspects from acoustic focusing, which helps you to placement the cells so that a sharp, centered impression is attained.

Sound waves help precise cell alignment, resulting in exceptional info good quality and ~10X speedier sample flow costs.

Software Be aware: Automated graphic analysis minimizes consumer-to-person variability in flow cytometry gating approaches

Leverage the power of Highly developed engineering and innovation to accelerate your discoveries. Have confidence in while in the Attune Flow Cytometer spouse and children to deliver the dependability, velocity, and accuracy that established your operate aside.

No wash/no lyse leukocyte analysis with imaging. Cells were acquired from 24-hour-aged blood by dilution in 1 mM EDTA ( Professional UX/UI permits consumers to educate AI types for expanded programs. (A) The “Educate Product” feature provides annotation applications and serious-time model teaching precision.

Gating on these prolonged parameters helps you to immediately and precisely establish populations of desire and make sure gating system with little or no manual overview. The Attune program features access to state-of-the-art equipment Understanding Investigation resources in just an expertly built person interface, making it extremely easy to use.

As the mobile line experienced Beforehand been analyzed on the Attune CytPix Flow Cytometer, the researchers went back to the images and ended up in the position to document the microbial infection a minimum of 5 days before.

The Attune CytPix Flow Cytometer enables end users to visualize samples during acquisition, verify the precision of gating approaches, and assist success with reputable documentation for publication purposes.

Below, we exhibit that by gating check here strategies utilizing image-based mostly quantitative parameters can distinguish interacting cells from coincident occasions to a lot more correctly evaluate interacting cells.